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bioarray high yield rna transcript labelling kit  (Enzo Biochem)

 
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    Structured Review

    Enzo Biochem bioarray high yield rna transcript labelling kit
    Bioarray High Yield Rna Transcript Labelling Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+yield+rna+transcript+labeling+kit/bioarray+high+yield+rna+transcript+labelling+kit/us12064465-1250-8-14
    Average 90 stars, based on 1 article reviews
    bioarray high yield rna transcript labelling kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Expression of cassini , a murine gamma-satellite sequence conserved in evolution, is regulated in normal and malignant hematopoietic cells
    Article Snippet: Briefly, RNA quality was first assessed using an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, California, USA) and the 28S/18S ratios of all of the samples were between 1.3 and 2. .. RNA was converted to cDNA with Superscript Choice for cDNA Synthesis (Invitrogen, Carlsbad, California, USA) and subsequently converted to biotinylated cRNA with an Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics, Farmingdale, New York, USA). .. After hybridization to the murine Mouse Gene 1.0 ST arrays (28,000 transcripts, Affymetrix, Santa Clara, California, USA), the gene chips were automatically washed and stained with streptavidin-phycoerythrin using a fluidics system.

    Article Title: Environment-mediated drug resistance in Bcr/Abl-positive acute lymphoblastic leukemia.
    Article Snippet: Briefly, RNA quality was first assessed using an Agilent Bioanalyzer (Agilent Technologies) and the 28S/18S ratios of all of the samples were between 1.3 and 2. .. RNA was converted to cDNA with Superscript Choice for cDNA Synthesis (Invitrogen) and subsequently converted to biotinylated cRNA with an Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics). .. After hybridization to the murine Mouse Gene 1.0 ST arrays (28,000 transcripts, Affymetrix), the gene chips were automatically washed and stained with streptavidinphycoerythrin using a fluidics system.

    Article Title: Adipocytes cause leukemia cell resistance to daunorubicin via oxidative stress response
    Article Snippet: RNA quality was first assessed using an Agilent Bioanalyzer (Agilent Technologies). .. RNA was then converted to cDNA with Superscript Choice for cDNA Synthesis (Invitrogen) and then converted to biotinylated cDNA using an Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics). .. GeneChip ® Mouse Gene ST Array (Affymetrix) was used for hybridization of cDNA.

    Labeling:

    Article Title: Expression of cassini , a murine gamma-satellite sequence conserved in evolution, is regulated in normal and malignant hematopoietic cells
    Article Snippet: Briefly, RNA quality was first assessed using an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, California, USA) and the 28S/18S ratios of all of the samples were between 1.3 and 2. .. RNA was converted to cDNA with Superscript Choice for cDNA Synthesis (Invitrogen, Carlsbad, California, USA) and subsequently converted to biotinylated cRNA with an Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics, Farmingdale, New York, USA). .. After hybridization to the murine Mouse Gene 1.0 ST arrays (28,000 transcripts, Affymetrix, Santa Clara, California, USA), the gene chips were automatically washed and stained with streptavidin-phycoerythrin using a fluidics system.

    Article Title: Theiler’s murine encephalomyelitis virus infection of astrocytes induces the expression of chemokines which attract activated but not resting T lymphocytes
    Article Snippet: .. The cDNA isolated was transcribed using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Biochem, New York, NY) with biotin-labeled UTP and CTP to produce biotin-labeled cRNA. ..

    Article Title: Theiler's virus infection provokes the overexpression of genes coding for the chemokine Ip10 (CXCL10) in SJL/J murine astrocytes, which can be inhibited by modulators of estrogen receptors.
    Article Snippet: Theiler’s murine encephalomyelitis virus (TMEV) induces demyelination in susceptible strains of mice (SJL/J) through an immunopathological process that is mediated by CD4 Th1 T cell.. These T cells are chemoattracted to the central nervous system by chemokines.. Hence, in this study, we focused on the production of the chemokine “interferongamma-inducible protein 10 kDa,” or IP-10/CXCL10, by cultured SJL/J mouse astrocytes infected with the BeAn strain of TMEV and its capacity to attract activated T cells.

    Article Title: Lipocalin-type prostaglandin D synthase regulates light-induced phase advance of the central circadian rhythm in mice
    Article Snippet: .. The second-round amplification was performed similarly, except that the Enzo High Yield RNA Transcript Labeling Kit (Enzo Diagnostics) was used to prepare biotin-labeled antisense RNA. .. The resulting antisense RNA was hybridized to the GeneChip Mouse Genome 430A array (Affymetrix) using standard methods.

    Article Title: Environment-mediated drug resistance in Bcr/Abl-positive acute lymphoblastic leukemia.
    Article Snippet: Briefly, RNA quality was first assessed using an Agilent Bioanalyzer (Agilent Technologies) and the 28S/18S ratios of all of the samples were between 1.3 and 2. .. RNA was converted to cDNA with Superscript Choice for cDNA Synthesis (Invitrogen) and subsequently converted to biotinylated cRNA with an Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics). .. After hybridization to the murine Mouse Gene 1.0 ST arrays (28,000 transcripts, Affymetrix), the gene chips were automatically washed and stained with streptavidinphycoerythrin using a fluidics system.

    Article Title: Gene expression profiling reveals effects of Cimicifuga racemosa (L.) NUTT. (black cohosh) on the estrogen receptor positive human breast cancer cell line MCF-7
    Article Snippet: .. From the ds-cDNA template biotin-labeled cRNA was made with the Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics, Farmingdale, NY). .. The labeled cRNA was purified following the Qiagen RNeasy Clean up protocol (Qiagen) and concentrated to 24 μl by ethanol precipitation.

    Article Title: Adipocytes cause leukemia cell resistance to daunorubicin via oxidative stress response
    Article Snippet: RNA quality was first assessed using an Agilent Bioanalyzer (Agilent Technologies). .. RNA was then converted to cDNA with Superscript Choice for cDNA Synthesis (Invitrogen) and then converted to biotinylated cDNA using an Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics). .. GeneChip ® Mouse Gene ST Array (Affymetrix) was used for hybridization of cDNA.

    Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
    Article Snippet: Briefly, 10 μg of total RNA was used to make double-stranded cDNA using reagents and protocols obtained from Invitrogen (Carlsbad, CA). .. Linear amplification was carried out by a modification of the Eberwine T7 method [ ], and biotin was incorporated into the cRNA using the Enzo High Yield RNA Transcript labeling Kit (EnzoDiagnostics, Farmingdale, NY). ..

    Isolation:

    Article Title: Theiler’s murine encephalomyelitis virus infection of astrocytes induces the expression of chemokines which attract activated but not resting T lymphocytes
    Article Snippet: .. The cDNA isolated was transcribed using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Biochem, New York, NY) with biotin-labeled UTP and CTP to produce biotin-labeled cRNA. ..

    Article Title: Theiler's virus infection provokes the overexpression of genes coding for the chemokine Ip10 (CXCL10) in SJL/J murine astrocytes, which can be inhibited by modulators of estrogen receptors.
    Article Snippet: Theiler’s murine encephalomyelitis virus (TMEV) induces demyelination in susceptible strains of mice (SJL/J) through an immunopathological process that is mediated by CD4 Th1 T cell.. These T cells are chemoattracted to the central nervous system by chemokines.. Hence, in this study, we focused on the production of the chemokine “interferongamma-inducible protein 10 kDa,” or IP-10/CXCL10, by cultured SJL/J mouse astrocytes infected with the BeAn strain of TMEV and its capacity to attract activated T cells.

    Amplification:

    Article Title: Lipocalin-type prostaglandin D synthase regulates light-induced phase advance of the central circadian rhythm in mice
    Article Snippet: .. The second-round amplification was performed similarly, except that the Enzo High Yield RNA Transcript Labeling Kit (Enzo Diagnostics) was used to prepare biotin-labeled antisense RNA. .. The resulting antisense RNA was hybridized to the GeneChip Mouse Genome 430A array (Affymetrix) using standard methods.

    Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
    Article Snippet: Briefly, 10 μg of total RNA was used to make double-stranded cDNA using reagents and protocols obtained from Invitrogen (Carlsbad, CA). .. Linear amplification was carried out by a modification of the Eberwine T7 method [ ], and biotin was incorporated into the cRNA using the Enzo High Yield RNA Transcript labeling Kit (EnzoDiagnostics, Farmingdale, NY). ..

    Modification:

    Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
    Article Snippet: Briefly, 10 μg of total RNA was used to make double-stranded cDNA using reagents and protocols obtained from Invitrogen (Carlsbad, CA). .. Linear amplification was carried out by a modification of the Eberwine T7 method [ ], and biotin was incorporated into the cRNA using the Enzo High Yield RNA Transcript labeling Kit (EnzoDiagnostics, Farmingdale, NY). ..



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